The transgenerational effect of thermosensing in plants. This project aims to understand how thermosensing mechanisms in plants result in transgenerational change, and potentially adaptation to climate. Exploiting the recent discovery of the thermosensor phytochrome B, this project will decipher the molecular cascade which, either through long-distance communication or through persistence of an epigenetic state in the cell lineage, could lead to a trans generational memory in plants helping with ....The transgenerational effect of thermosensing in plants. This project aims to understand how thermosensing mechanisms in plants result in transgenerational change, and potentially adaptation to climate. Exploiting the recent discovery of the thermosensor phytochrome B, this project will decipher the molecular cascade which, either through long-distance communication or through persistence of an epigenetic state in the cell lineage, could lead to a trans generational memory in plants helping with climate adaptation. This project will unravel novel molecular mechanisms, which have the potential to pave the way for designing new climate-proofing solutions to cope with temperature uncertainty.Read moreRead less
Genome-wide determination of Puccinia psidii s.l. rust resistance in eucalypts. Recently, guava rust was detected in Australia, posing significant risks to native flora, plantations, and timber exports. Scientists from The University of Melbourne and Victorian Department of Primary Industries together with tree breeders, forest growers and forest managers aim to use tree genomics rust resistance breeding to enable management and operational responses and inform policy development.
Identification of Biological pathways regulated by circular RNAs. Circular RNAs (circRNAs) are a, recently discovered molecule. circRNAs are highly abundant and expressed in a tissue and disease specific manner. Yet, currently the understanding of how circRNAs regulate biological processes is very poor. This project aims to use pooled shRNA libraries to screen a large panel of cell lines and systematically identify cellular activities that are regulated by circRNAs. The expected outcome of this ....Identification of Biological pathways regulated by circular RNAs. Circular RNAs (circRNAs) are a, recently discovered molecule. circRNAs are highly abundant and expressed in a tissue and disease specific manner. Yet, currently the understanding of how circRNAs regulate biological processes is very poor. This project aims to use pooled shRNA libraries to screen a large panel of cell lines and systematically identify cellular activities that are regulated by circRNAs. The expected outcome of this study will be a catalogue of functionally active circRNAs. Over the past decades, the wealth of knowledge on the function of linear mRNAs has had a significant impact on medicine and agriculture. Similarly understanding how circRNAs regulate cellular activities may have an analogous impact on humans.Read moreRead less
Targeting the genome and epigenome of the exercising skeletal muscle. This project aims is to discover epigenetic and genetic biomarkers that predict fitness changes, following exercise intervention. Individuals are remarkably variable in their responses to exercise interventions, and a large portion of these responses is attributed to genetics, and epigenetics (the effect of the environment on the expression of genes). Using controlled exercise training as a model, this project expects to disco ....Targeting the genome and epigenome of the exercising skeletal muscle. This project aims is to discover epigenetic and genetic biomarkers that predict fitness changes, following exercise intervention. Individuals are remarkably variable in their responses to exercise interventions, and a large portion of these responses is attributed to genetics, and epigenetics (the effect of the environment on the expression of genes). Using controlled exercise training as a model, this project expects to discover epigenetic and genomic markers in skeletal muscle predictive of exercise adaptations. This will contribute to the development and future delivery of targeted and personalised exercise programs for the general population. This has important implications for improving health in the Australian population.Read moreRead less
HEN1 is a regulator of piRNA metabolism, transcriptional regulation and mammalian male fertility. This project is to define the biochemistry of a previously uncharacterized protein in male fertility using a unique mouse model and innovative DNA and protein technologies. This project will define a novel, and essential, pathway for male fertility and may ultimately have relevance to the maintenance of health or improving fertility.
Genomics of temperature response in plants. Climate change is predicted to have negative impacts on Australian agriculture. This project will use genomic tools to uncover biological mechanisms for plant response to temperature that will help design crop varieties that are more tolerant to higher temperatures.
The role of non-coding RNAs in T cell development. The goal of this project is to discover the genes responsible for the development of a healthy immune system. To achieve this goal, a battery of next generation genomics technologies are being applied for the discovery of new genes and to study their function.
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE140100114
Funder
Australian Research Council
Funding Amount
$560,000.00
Summary
High Throughput Cell Genomics Centre. High throughput cell genomics centre: This project will establish a high throughput cell genomics centre comprising a Fluidigm C1™ Single-Cell AutoPrep and BioMark™ HD system providing researchers with the most innovative approach to single cell and small population analyses. The instruments will enable the unique capability to conduct single cell transcriptome analysis and high throughput gene expression, SNP genotyping and copy number variation analysis as ....High Throughput Cell Genomics Centre. High throughput cell genomics centre: This project will establish a high throughput cell genomics centre comprising a Fluidigm C1™ Single-Cell AutoPrep and BioMark™ HD system providing researchers with the most innovative approach to single cell and small population analyses. The instruments will enable the unique capability to conduct single cell transcriptome analysis and high throughput gene expression, SNP genotyping and copy number variation analysis as well as validation of next generation sequencing data. The information generated is crucial to advancing knowledge in important research fields including infection and immunity, regenerative medicine, immune responses, biomarker discovery, drug discovery, biotechnology and agriculture.Read moreRead less
Evolution and function of mammalian sex chromosomes. Research on iconic Australian mammals has profoundly reshaped our understanding of reproductive biology and sex chromosome evolution. In this project we combine unique expertise, international collaboration and novel genetic information about Australia's unique egg-laying mammals (echidna and platypus) to investigate major aspects of reproduction. This work will address fundamental aspects of sex chromosome biology and advance our understandin ....Evolution and function of mammalian sex chromosomes. Research on iconic Australian mammals has profoundly reshaped our understanding of reproductive biology and sex chromosome evolution. In this project we combine unique expertise, international collaboration and novel genetic information about Australia's unique egg-laying mammals (echidna and platypus) to investigate major aspects of reproduction. This work will address fundamental aspects of sex chromosome biology and advance our understanding of mammalian reproduction. The knowledge gained will have application in captive breeding and conservation of these extraordinary Australian mammals. The project also provides opportunity to train research students in cutting edge molecular biology and informatics.Read moreRead less
Alternative splicing in apicomplexan parasites. The project intends to provide a detailed picture of how alternative splicing is regulated in four biologically diverse apicomplexan parasites, and to explain why parasites need this molecular trick to survive. Alternative splicing is an important means by which organisms increase the diversity of proteins encoded by their genome. Although this mechanism is well studied in humans, little is known about the extent of this phenomenon in other organis ....Alternative splicing in apicomplexan parasites. The project intends to provide a detailed picture of how alternative splicing is regulated in four biologically diverse apicomplexan parasites, and to explain why parasites need this molecular trick to survive. Alternative splicing is an important means by which organisms increase the diversity of proteins encoded by their genome. Although this mechanism is well studied in humans, little is known about the extent of this phenomenon in other organisms, nor how the process is regulated. The project plans to test if alternative splicing is required to transition between different life stages, just as alternative splicing is required for tissue differentiation in animals, and describe how alternative splicing contributes to apicomplexan proteome diversity.Read moreRead less