Evolutionary algorithms for problems in functional genomics data analysis. Skin cancer has a high incidence in the Australian population. Schizophrenia is a psychiatric disorder that affects a significant proportion of the population worldwide. Both illnesses have genetic roots and can be triggered by environmental factors. We will uncover genetic relationship to disease and their responses to environmental conditions using computational methods and mathematical algorithms that can aid in the de ....Evolutionary algorithms for problems in functional genomics data analysis. Skin cancer has a high incidence in the Australian population. Schizophrenia is a psychiatric disorder that affects a significant proportion of the population worldwide. Both illnesses have genetic roots and can be triggered by environmental factors. We will uncover genetic relationship to disease and their responses to environmental conditions using computational methods and mathematical algorithms that can aid in the determination of function, especially in disease states. Understanding the complex genetic interactions that trigger these illnesses would give great benefits in preventive health care, skin cancer and schizophrenia genetic basis, and may lay the ground for building new methods for "personalized medicine".
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Gene Expression Profiling and de novo Transcriptome Sequencing using Geneballs. The purpose of the project is to demonstrate that bead-based technology can be used in applications that currently require DNA hybridisation in order to overcome existing deficiencies in microarray technology. By providing the capability to quickly and efficiently produce, screen and utilize biomolecule libraries of nearly unlimited size, this technology provides the key to unlock the power of genomics and proteomics ....Gene Expression Profiling and de novo Transcriptome Sequencing using Geneballs. The purpose of the project is to demonstrate that bead-based technology can be used in applications that currently require DNA hybridisation in order to overcome existing deficiencies in microarray technology. By providing the capability to quickly and efficiently produce, screen and utilize biomolecule libraries of nearly unlimited size, this technology provides the key to unlock the power of genomics and proteomics for use in real world applications. The project has two aspects. First, relatively small directed cDNA-bead libraries will be compared to known low-density cDNA microarrays to validate the technique for utility in gene expression profiling. Secondly, large libraries containing short oligonucleotide sequences will be used for de novo sequencing of a complete transcriptome. Proof-of-concept in either case will pave the way for many genomic applications and catapult the technology to 'blockbuster' status.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0453920
Funder
Australian Research Council
Funding Amount
$108,680.00
Summary
Molecular diagnostics based on real-time polymerase chain reactions for emerging tropical infectious diseases aimed at protecting Australia from invasive diseases. The project aims to use the technique of real-time polymerase chain reaction to rapidly detect and quantify the organisms associated with emerging and re-emerging infectious diseases of man and animals. It will also be used to determine related gene expression.
The equipment will be used to support a wide range of projects that req ....Molecular diagnostics based on real-time polymerase chain reactions for emerging tropical infectious diseases aimed at protecting Australia from invasive diseases. The project aims to use the technique of real-time polymerase chain reaction to rapidly detect and quantify the organisms associated with emerging and re-emerging infectious diseases of man and animals. It will also be used to determine related gene expression.
The equipment will be used to support a wide range of projects that require the detection of specific RNA or DNA and it will allow the rapid, cost effective and efficient processing of either RNA or DNA from large numbers of samples. Minor variations in organisms will be detected using this equipment.
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Classification of Microarray Gene-Expression Data. The broad aim is to provide statistical methodology for the classification of microarray gene-expression data. Microarrays are part of a new biotechnology that allows the monitoring of expression levels for thousands of genes simultaneously. The explosion in microarrays has produced massive quantities of data that require new statistical techniques for analysis in order to exploit their enormous scientific potential. One of the main uses of ....Classification of Microarray Gene-Expression Data. The broad aim is to provide statistical methodology for the classification of microarray gene-expression data. Microarrays are part of a new biotechnology that allows the monitoring of expression levels for thousands of genes simultaneously. The explosion in microarrays has produced massive quantities of data that require new statistical techniques for analysis in order to exploit their enormous scientific potential. One of the main uses of the methodology to be developed is to expedite the discovery of new subclasses of diseases. Another is to provide prediction rules for the diagnosis and treatment of diseases.Read moreRead less
Automatic detection of the circle of Willis in neuro-images using multi-scale gradient calculation and knowledge-based genetic algorithms. Stroke is the third most common cause of death and a major contributor to long term disability in Australia. The most efficient way of preventing stroke from happening is to detect related symptoms early. The group of cerebral blood vessels that closely related to strokes is the circle of Willis (CoW). We build a system that can automatically detect and quan ....Automatic detection of the circle of Willis in neuro-images using multi-scale gradient calculation and knowledge-based genetic algorithms. Stroke is the third most common cause of death and a major contributor to long term disability in Australia. The most efficient way of preventing stroke from happening is to detect related symptoms early. The group of cerebral blood vessels that closely related to strokes is the circle of Willis (CoW). We build a system that can automatically detect and quantify CoW in neuroimages, providing ways of preventing strokes from happening. The project will enhance Australia¡¯s leading position in promoting and maintaining good health, especially in preventive healthcare.Read moreRead less
Heterodensity neuroimaging techniques for spatiotemporal identification and localization. Australia is at the forefront of research that is taking brain imaging and interfacing out of the lab and into the field. The technology being developed is spawning applications in areas as diverse as health, education, defense and national security, and will lead to resolution of longstanding theoretical debates as well as enabling new breakthroughs in understanding the biological concomitants of psycholo ....Heterodensity neuroimaging techniques for spatiotemporal identification and localization. Australia is at the forefront of research that is taking brain imaging and interfacing out of the lab and into the field. The technology being developed is spawning applications in areas as diverse as health, education, defense and national security, and will lead to resolution of longstanding theoretical debates as well as enabling new breakthroughs in understanding the biological concomitants of psychological function. This is significant given the push to develop biometrics of psychological function with enhanced sensitivity and specificity in clinical diagnosis, along with defense applications in evaluation of critical personnel and training. It also opens up a range of applications for mental control of remote or prosthetic devices.Read moreRead less
Sequencing the mammalian transcriptome in toto. The mammalian genome projects have provided a huge leap forward for biological research by giving the framework to study genes on a global scale. This project will provide human genome project scale information for every major tissue in mammals and will provide the research community with a world class resource to further genomic research. It will also investigate parts of gene regulation which have been invisible to researchers in the past. Discov ....Sequencing the mammalian transcriptome in toto. The mammalian genome projects have provided a huge leap forward for biological research by giving the framework to study genes on a global scale. This project will provide human genome project scale information for every major tissue in mammals and will provide the research community with a world class resource to further genomic research. It will also investigate parts of gene regulation which have been invisible to researchers in the past. Discovering these rules will provide novel insights in pathology with a genetic component and provide a further boost to biotechnological approaches to obtain expression of specific sets of genes in model systems. Read moreRead less
An MEG (brain imaging) system to study cognitive processing in children. This project introduces the world's first brain imaging system to study cognitive processing in children, using magnetoencephalography (MEG). MEG offers precise measurement of brain activities using a non-invasive, non-contact method. MEG is an ideal brain imaging device for use with children and with special clinical populations. It promises to be valuable (a) for the identification of cortical functions of the human brain ....An MEG (brain imaging) system to study cognitive processing in children. This project introduces the world's first brain imaging system to study cognitive processing in children, using magnetoencephalography (MEG). MEG offers precise measurement of brain activities using a non-invasive, non-contact method. MEG is an ideal brain imaging device for use with children and with special clinical populations. It promises to be valuable (a) for the identification of cortical functions of the human brain prior to surgery, (b) for the diagnosis of loci for developmental and acquired brain disorders, (c) in determining pharmaceutical effects on children, and (d) in the assessment of recovery of sensory and cognitive functions following brain injury. Read moreRead less
Intelligent Image Processing Techniques for Novel Biomarker Discovery. This project will make an impact on Australia's international research profile by seeking a solution to a worldwide challenging problem in biomarker discovery for the detection of diseases at an early stage which requires the incorporation of the skills and knowledge from biology, medicine, engineering, computer science, and information technology. The successful outcomes of this research will make an impact on Australia's e ....Intelligent Image Processing Techniques for Novel Biomarker Discovery. This project will make an impact on Australia's international research profile by seeking a solution to a worldwide challenging problem in biomarker discovery for the detection of diseases at an early stage which requires the incorporation of the skills and knowledge from biology, medicine, engineering, computer science, and information technology. The successful outcomes of this research will make an impact on Australia's engagement in using advanced image analysis and intelligent methods for the emerging research and development of targeted drug discovery. Read moreRead less
The Role of High-Frequency Antigenic Variation in The Pathogenesis of Mycoplasma infection. The main goal of the proposed project is to understand the molecular mechanisms of phase/antigenic variation and its effects on mycoplasma pathogenesis. In this context I will use the well-characterised Mycoplasma synoviae haemagglutinin, MSPA, to establish the role of its phase-variable expression in the type and extent of M. synoviae disease. Additionally, the relationship between MSPA phase variation a ....The Role of High-Frequency Antigenic Variation in The Pathogenesis of Mycoplasma infection. The main goal of the proposed project is to understand the molecular mechanisms of phase/antigenic variation and its effects on mycoplasma pathogenesis. In this context I will use the well-characterised Mycoplasma synoviae haemagglutinin, MSPA, to establish the role of its phase-variable expression in the type and extent of M. synoviae disease. Additionally, the relationship between MSPA phase variation and gene rearrangements in the MSPB-encoding gene will be elucidated. The results will contribute to our understanding of the pathogenesis of bacterial disease and of the evolution of pathogenic mechanisms in bacterial pathogens.Read moreRead less