Suction pipette measurements of mammalian rod photoreceptor recovery following intense bleaching exposures. The aim of this project is to discover the events and processes that prevent retinal photoreceptors from recovering instantaneously following the cessation of exposure to extremely bright illumination. Recordings will be made from single rod photoreceptors cells isolated from the mammalian retina. The work will uncover the relative roles of the 'photoproducts' created when rhodopsin abso ....Suction pipette measurements of mammalian rod photoreceptor recovery following intense bleaching exposures. The aim of this project is to discover the events and processes that prevent retinal photoreceptors from recovering instantaneously following the cessation of exposure to extremely bright illumination. Recordings will be made from single rod photoreceptors cells isolated from the mammalian retina. The work will uncover the relative roles of the 'photoproducts' created when rhodopsin absorbs light: e.g. intermediates such as metarhodopsin and opsin. The molecular knowledge obtained will help us to understand why it is that the visual system recovers so slowly after the eye has experienced very intense light.Read moreRead less
The first stage of vision: transduction and adaptation in retinal photoreceptors. The project aims to provide a detailed understanding of the molecular steps involved in the first stage of vision - the conversion of light into a neural signal in the rod and cone photoreceptors of the retina. The significance of this is that it will explain the initial events that enable us to see, and will help explain the deficits that occur when the process fails. The outcome will be a comprehensive understand ....The first stage of vision: transduction and adaptation in retinal photoreceptors. The project aims to provide a detailed understanding of the molecular steps involved in the first stage of vision - the conversion of light into a neural signal in the rod and cone photoreceptors of the retina. The significance of this is that it will explain the initial events that enable us to see, and will help explain the deficits that occur when the process fails. The outcome will be a comprehensive understanding of how our photoreceptors respond with extreme sensitivity, yet great rapidity, and over an enormous range of light intensities, thus endowing us with our remarkable sense of vision.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0989408
Funder
Australian Research Council
Funding Amount
$150,000.00
Summary
A multiple-ion membrane inlet mass spectrometer. There is a compelling need for a modern isotope-ratio mass spectrometer to facilitate a range of innovative studies of chemical and biochemical processes. Mass spectrometers operated with a thin permeable membrane over a vacuum inlet allow small gas molecules to be continuously monitored inside specific gas or liquid phase sample chambers. The proposed instrumentation will enable simultaneous and highly sensitive measurement of 10 different isot ....A multiple-ion membrane inlet mass spectrometer. There is a compelling need for a modern isotope-ratio mass spectrometer to facilitate a range of innovative studies of chemical and biochemical processes. Mass spectrometers operated with a thin permeable membrane over a vacuum inlet allow small gas molecules to be continuously monitored inside specific gas or liquid phase sample chambers. The proposed instrumentation will enable simultaneous and highly sensitive measurement of 10 different isotopic species and the capability for detection of hydrogen. This equipment will provide the many researchers and students with opportunities to determine isotopic signatures of isolated chemical reactions through to complex biochemical systems of whole living cells.Read moreRead less
Mineral content of leaves and the ratio of water loss to carbon gain: environmental and genetic controls and comparison with stable isotopic measures. The ash content of leaves has promise as a cheap screen of water-use efficiency or of 'vigour' in crop plants, but the underlying mechanisms are not understood. The underlying science is at the intersection of plant growth, water use and nutrition. This project will aid breeders in understanding the conditions under which the screen may work.
Estimating And Alleviating The Impacts Of Age-Related Sensory Decline
Funder
National Health and Medical Research Council
Funding Amount
$323,767.00
Summary
Sensory loss and dementia disproportionately affect older adults, often co-occur, and are the two leading contributors to disability burden among older Australians. This research will investigate the consequences that hearing and vision loss have for older adult health and wellbeing, informing strategies to reduce the disability burden of age-related sensory loss. These impacts include cognitive decline and dementia, mental health, quality of life, disability and independent living.
Avoiding coral bleaching: investigation into the repair of damaged photosynthetic machinery in symbiotic algae (symbiodinium) within corals. Photosynthesis in symbiotic algae within corals is essential for a healthy alga-coral symbiotic relationship. This project will provide new insights into how symbiotic algae maintain higher photosynthetic performance in corals through elucidating the mechanism associated with the repair of photodamaged photosynthetic machinery.
A step change in modeling leaf respiration-photosynthesis relationships . This project aims to use innovative, high-throughput technologies to develop a novel framework that links daytime photosynthesis and starch/amino acid mobilisation to variations in night-time leaf respiration. Variations in leaf respiration can have large impacts on ecosystem functioning and the Earth’s climate. Although advances have been made in respiration modelling, current models are unable to predict dynamic, day-to- ....A step change in modeling leaf respiration-photosynthesis relationships . This project aims to use innovative, high-throughput technologies to develop a novel framework that links daytime photosynthesis and starch/amino acid mobilisation to variations in night-time leaf respiration. Variations in leaf respiration can have large impacts on ecosystem functioning and the Earth’s climate. Although advances have been made in respiration modelling, current models are unable to predict dynamic, day-to-day variations in respiratory rates. Expected outcomes include equations that predict daily variations in night-time leaf respiration for environments across Australia and overseas. Benefits to planners include the ability to more accurately model vegetation-atmosphere carbon exchange and future changes in climate. Read moreRead less
The future of forests under climatic stress. This project aims to measure the vulnerability of forest trees to more extreme drought as global temperatures inevitably rise. Australian forests face the immediate threat of increased mortality associated with intensifying drought stress in the future. Understanding the magnitude of this threat is of the utmost urgency. This project aims to predict future mortality of forest communities in Australia and worldwide using recent breakthroughs enabling t ....The future of forests under climatic stress. This project aims to measure the vulnerability of forest trees to more extreme drought as global temperatures inevitably rise. Australian forests face the immediate threat of increased mortality associated with intensifying drought stress in the future. Understanding the magnitude of this threat is of the utmost urgency. This project aims to predict future mortality of forest communities in Australia and worldwide using recent breakthroughs enabling the rapid quantification of lethal stress in trees. This new understanding will provide a basis upon which to make far-reaching decisions about land management, conservation and restoration.Read moreRead less
Involvement of cell coupling in vascular function: Development of a computational model. Gap junctions are intercellular channels which enable the production of coordinated responses in multicellular tissues and organs. Blood vessels are comprised of endothelial cells surrounded by smooth muscle cells and gap junctions exist within and between these layers. The present proposal will determine the fundamental role of gap junctions in regulating blood flow and blood pressure. Our data will enable ....Involvement of cell coupling in vascular function: Development of a computational model. Gap junctions are intercellular channels which enable the production of coordinated responses in multicellular tissues and organs. Blood vessels are comprised of endothelial cells surrounded by smooth muscle cells and gap junctions exist within and between these layers. The present proposal will determine the fundamental role of gap junctions in regulating blood flow and blood pressure. Our data will enable us to develop a computational model of the vascular wall and so predict how changes in electrical properties, as occur during pressure changes, can influence blood flow. Since ageing is accompanied by an increase in blood pressure, our results will contribute to a better understanding of blood flow regulation in our ageing population.Read moreRead less
Structural Determinants of an Intracellular Calcium Store. Understanding the molecular interactions between key proteins in calcium signalling in muscle and the heart will allow calcium signalling to be used as a platform for a variety of purposes. These include reducing the debilitating effects of changes in calcium signalling and muscle performance in aging and in genetically- or drug-induced disorders. The project will have benefits for Australian biotechnology since it will facilitate the de ....Structural Determinants of an Intracellular Calcium Store. Understanding the molecular interactions between key proteins in calcium signalling in muscle and the heart will allow calcium signalling to be used as a platform for a variety of purposes. These include reducing the debilitating effects of changes in calcium signalling and muscle performance in aging and in genetically- or drug-induced disorders. The project will have benefits for Australian biotechnology since it will facilitate the design of novel compounds for treating muscle disorders in animals and humans, for improving meat quality and for use as insecticides. The project will facilitate graduate and undergraduate training in basic science with exposure to biotechnology, through our commercial partner Biotron.Read moreRead less