Role of mRNA polyadenylation control in gene expression. Several benefits would come from a more complete understanding of the function of the messenger RNA poly(A) tail. It is frequently targeted by mechanisms that control cellular protein synthesis. This is most evident in developmental biology, where tail length control regulates maternal mRNA expression. Our previous work suggests that it has much wider importance for cellular function than previously thought and thus its study will produce ....Role of mRNA polyadenylation control in gene expression. Several benefits would come from a more complete understanding of the function of the messenger RNA poly(A) tail. It is frequently targeted by mechanisms that control cellular protein synthesis. This is most evident in developmental biology, where tail length control regulates maternal mRNA expression. Our previous work suggests that it has much wider importance for cellular function than previously thought and thus its study will produce knowledge of broad relevance to modern life sciences and its applications in medicine and biotechnology. Finally, a better understanding of yeast cellular biology is of benefit to the food and biotechnology sector of industry.Read moreRead less
Application of genome-wide transcriptional analysis to identifying genetic markers for industrial fermentation processes. This project aims to identify yeast genes involved in the response of commercial strains to stress, to determine their role in fermentation and the genetic pathways through which they operate. The ultimate goals are to determine the impact on fermentation activity of stress, and to develop predictive methods for assessing such conditions. This has significance since stress ....Application of genome-wide transcriptional analysis to identifying genetic markers for industrial fermentation processes. This project aims to identify yeast genes involved in the response of commercial strains to stress, to determine their role in fermentation and the genetic pathways through which they operate. The ultimate goals are to determine the impact on fermentation activity of stress, and to develop predictive methods for assessing such conditions. This has significance since stress during fermentation represents a significant commercial loss. The outcomes of this work will be a better understanding of how yeast responds to stress, and the identification of genes that can be used by the commercial partner to monitor and ensure fermentation efficiency.Read moreRead less
Determining the sequence of events during eukaryotic translation initiation. Multiple benefits will arise from a more complete understanding of translation initiation. It is a frequent target for mechanisms that control gene expression and its dys-regulation is associated with human disease. For example, this is evident in cancer biology, since altered translation is a frequent cause of tumorigenesis. Translation is of universal importance for cellular function and knowledge of how it works is c ....Determining the sequence of events during eukaryotic translation initiation. Multiple benefits will arise from a more complete understanding of translation initiation. It is a frequent target for mechanisms that control gene expression and its dys-regulation is associated with human disease. For example, this is evident in cancer biology, since altered translation is a frequent cause of tumorigenesis. Translation is of universal importance for cellular function and knowledge of how it works is central to modern life sciences and its application to medical and biotechnological problems. Finally, a better understanding of yeast cellular biology is of benefit to the food and biotechnology sector of industry.Read moreRead less
Understanding the differentiation of the endocardium. The project aims to understand the genetic regulation of endocardial development. The heart is essential for survival, its beat the indicator of life. The endocardium, the heart’s inner lining, is required for signalling during heart development and is a major component of the valves, septa and trabeculae. Despite its indispensable role, little is known about how it forms or develops. This project integrates two complementary approaches that ....Understanding the differentiation of the endocardium. The project aims to understand the genetic regulation of endocardial development. The heart is essential for survival, its beat the indicator of life. The endocardium, the heart’s inner lining, is required for signalling during heart development and is a major component of the valves, septa and trabeculae. Despite its indispensable role, little is known about how it forms or develops. This project integrates two complementary approaches that have identified the earliest marker of endocardial differentiation and devised the method to make endocardium from stem cells. Knowledge from this work will inform future research into growing and regenerating damaged tissue.Read moreRead less
Kruppel-like factors and the methylome. This project aims to test the hypothesis that the KLF/SP family of transcription factors work in part via dynamic interactions with methylated cytosine nucleotides in DNA. This is fundamental to their function as pioneer factors in reprograming and their ability to co-ordinate differentiation and organogenesis. Conversely, dynamic changes in methylation status engage or disengage new regulatory elements in the genome via recruitment of KLF/SP family protei ....Kruppel-like factors and the methylome. This project aims to test the hypothesis that the KLF/SP family of transcription factors work in part via dynamic interactions with methylated cytosine nucleotides in DNA. This is fundamental to their function as pioneer factors in reprograming and their ability to co-ordinate differentiation and organogenesis. Conversely, dynamic changes in methylation status engage or disengage new regulatory elements in the genome via recruitment of KLF/SP family proteins as specific effectors. This project will address a new paradigm in genetics that is likely to underpin development.Read moreRead less
How do transcription factors control cell fate transitions? The aim of this project is to determine how transcription factors control cellular identity, which is relevant to many biological processes including embryogenesis, cellular reprogramming and differentiation. Innovative genomic tools will be combined with various in vitro cellular conversion systems to generate fundamental mechanistic insight into how transcription factors mediate these identity changes. The knowledge gained from this w ....How do transcription factors control cell fate transitions? The aim of this project is to determine how transcription factors control cellular identity, which is relevant to many biological processes including embryogenesis, cellular reprogramming and differentiation. Innovative genomic tools will be combined with various in vitro cellular conversion systems to generate fundamental mechanistic insight into how transcription factors mediate these identity changes. The knowledge gained from this work will allow us to answer standing fundamental questions in regards to cell fate control and the biochemistry of transcription factors, which in turn will aid in the development of novel gene regulation technologies applicable to a myriad of fields and industries.Read moreRead less
Unveiling the epigenome dynamics through the pluripotency continuum. This project aims to utilise stem cells and genomics based technologies, in combination with new computational algorithms to dissect the fundamental molecular events that drive the first steps during development. The project is expected to unveil the basic mechanisms underpinning how genes driving the developmental master plan are controlled in cells that have the capacity to give rise to the whole organism and placenta. The kn ....Unveiling the epigenome dynamics through the pluripotency continuum. This project aims to utilise stem cells and genomics based technologies, in combination with new computational algorithms to dissect the fundamental molecular events that drive the first steps during development. The project is expected to unveil the basic mechanisms underpinning how genes driving the developmental master plan are controlled in cells that have the capacity to give rise to the whole organism and placenta. The knowledge gained from this work will inform and guide future novel approaches, such as in assisted reproductive technologies or regenerative medicine.Read moreRead less